Effects of bone marrow mesenchymal stem cells transplantation on the neurotrophic factor expression and cell apoptosis in the rats with spina bifida aperta

VernacularTitle:胚胎期骨髓间充质干细胞移植对先天性脊柱裂大鼠脊髓NGF和BDNF的表达及细胞凋亡的影响
Author: Jianing MIAO ; Bo LIU ; Di WU ; Guifeng ZHAO ; Hui LI ; Zhengwei YUAN
Keywords: Spina bifida aperta; Bene marrow mesenchymal cell; Apoptosis; Neurotrophic factor
From: International Journal of Pediatrics 2017;44(3):210-213,封3
CountryChina
Language:Chinese
Abstract: Objective To observe the effects of bone marrow mesenchymal stem cells (BMSC) transplantation on the expression of nerve growth factor(NGF) and brain derived neurotrophic factor(BDNF) in the rat spinal cord with spina bifida,and to investigate the change in cell apoptosis after BMSC transplantation.Methods Spina bifida aperta was induced with a single intragastric injection of all-trans retinoic acid,then the BMSC was microinjected into spina cord of rat embryos on embryo 16 d(E16),BDNF and NGF were tested by immunofluorescence staining,and TUNEL assay were used for investigating cell apoptosis.Results Transplantation of BMSC enhanced the expression of NGF and BDNF,and reduced cell apoptosis in the defective site of spinal cord.Conclusion The transplantation of BMSC may improve the microenvironment of spinal cord and repair the neurological defects by enhancing the expression of neurotrophic factor and reducing the cell apoptosis.