Purification Method of Ultrasonic Emulsification Combined with Ultrafiltration Membrane Separation for Flavonoids in Cacumen Platycladi
- VernacularTitle:均质法联合超滤膜纯化侧柏叶黄酮成分的方法初探
- Author:
Xianyi WANG
;
Ruihai LI
;
Tianzhu JIA
- Keywords:
Cacumen Platycladi;
Flavonoids;
Orthogonal experiment;
Ultrasonic emulsification;
Ultrafiltration membrane
- From:
China Pharmacist
2017;20(4):661-663,667
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain flavonoids of Cacumen Platycladi with high purity by using the method of ultrasonic emulsification combined with ultrafiltration membrane separation.Methods:Seven compounds including myricetrin as the reference substances,an HPLC method was used for the content determination.Using the single factor experiments,ultrasound,microwave,reflux and ultrasonic emulsification were compared,and using the orthogonal experiments,the ultrasonic emulsification time,ethanol concentration,solid-liquid ratio and times were studied.Biomax-5 membrane was adopted to improve the membrane separation.Results:The single factor experiments showed the homogeneous extraction method with the highest contents.The orthogonal experiments showed the optimal extracting conditions as follows:the ultrasonic emulsification time was 15 min,the ethanol concentration was 50%,the material-liquid ratio was 1∶10,and the extraction times was 3.The conditions of ultrafiltration membrane separation were as follows:the flow rate was 1.5 L·min-1,and the membrane separation pressure was 0.8 kg.Conclusion:The combination of ultrasonic emulsification and ultrafiltration membrane separation is feasible in the extraction and purification of flavonoids in Cacumen Platycladi,and the product is with high purity,suggesting the method has good application prospect.
