Isolation and Identification of Platelet by Stepped Centrifugal Method
10.3969/j.issn.1671-7414.2017.02.037
- VernacularTitle:阶梯式离心法血小板提取及鉴定方法的建立
- Author:
Xiangli YANG
;
Dianpeng WANG
;
Peimao LI
;
Zhimin ZHANG
;
Lidan DENG
;
Yanfang ZHANG
;
Fei ZHOU
;
Xianqing HUANG
- Keywords:
platelets;
isolaion;
identification
- From:
Journal of Modern Laboratory Medicine
2017;32(2):135-137,140
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the isolation and identification of platelets.Methods 10 healthy volunteers were selected to collect the EDTA anticoagulant venous blood of 3 tubes,each tube was 2 ml,which was divided into the whole blood cell tube,platelet rich plasma (control group),and stepped centrifugal platelet extract (experiment group).Platelet was isolated by simple centrifugation method(PRP) and stepped centrifugal method.The two groups were full blood count and analyzed by microscopic morphology and platelet activity test.Leukocyte specific HGB gene and platelet mitochondrial ND1 gene content was analyzed by real time PCR.Results Platelets were extracted and detected in control group and experimental group.Platelets were found and white blood cells and red blood cells were not remained in experimental group.Platelets and sporadic white blood cells were found in control group.The platelet pick up rate of experiment group was significantly higher than control group,the difference was statistically significant.Experimental gene content HGB of experiment group was significantly lower than control group,the difference was statistically significant (t=-3.281,-2.865,P＜0.05).ND1 gene content of experiment group higher than the control group,the difference was not statistically significant.There was no significant difference for platelet activity test between experimental group and control group (t=-0.046,-0.799,P＞ 0.05).Conclusion A isolation and identification method of stepped centrifugal platelet was established.The method can be used for the study of platelet gene and the functional analysis of platelets.
