Effects of Hongqi formular combined with exercise preconditioning against myocardial ischemic reperfusion injury in rats
10.3969/j.issn.1001-1242.2017.04.011
- VernacularTitle:红芪复方联合运动预处理对大鼠心肌缺血再灌注损伤保护作用
- Author:
Qiang WANG
;
Hongfei SHI
- Keywords:
myocardial ischemic reperfusion;
exercise;
hongqi formula;
preconditioning
- From:
Chinese Journal of Rehabilitation Medicine
2017;32(4):434-439,463
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects and mechanisms of Hongqi formular combined with exercise preconditioning against myocardial ischemic reperfusion injury in rats.Method:Fifty health male SD rats were randomly divided into sham,IR,EP,HP and EHP group.After establishing of EP animal model,the rats were ligated ramus descendens coronary artery to induce myocardial ischemic reperfusion model.The serum cardiac troponin Ⅰ (cTn Ⅰ),myocardium IL-6,TNF-α were detected by enzyme linked immunosorbent assay (ELISA).The myocardium MDA was detected by chemical colorimetry method.The myocardium SOD was detected by xanthine hydrocarbonylation.The serum ET,6-k-PGF1α,TXB2 density were detected by radioimmunoassay,and the myocardium Ca2+ density was detected by atom absorption spectrophotometry.Result:①As compared with the group IR,the serum cTn Ⅰ decreased significantly in groups EP,HP and EHP;As compared with the group EHP,the cTn Ⅰ level in serum was increased in groups EP and HP.②As compared with the group IR,the Ca2+ level in myocardial sarcoplasmic reticulum was decreased significantly in groups EP,HP and EHP;As compared with the group EHP,the Ca2+ level in myocardial sarcoplasmic reticulum was decreased significantly in group EP.③As compared with the group IR,the ET in serum were decreased significantly in groups EP,HP and EHP;As compared with the group EHP,the ET,TXB2 in serum were increased significantly in groups EP and the 6-k-PGF1αin serum were decreased significantly in groups EP.④As compared with the group IR,the IL-6 and TNF-α in cardiac muscle were decreased in groups EP,HP and EHP;As compared with the group EHP,the IL-6 and TNF-α in cardiac muscle were increased in groups EP and HP.⑤As compared with the group IR,the SOD in cardiac muscle was increased significantly in groups EP,HP and EHP and the MDA in cardiac muscle was decreased significantly in groups EP,HP and EHP;As compared with the group EHP,the SOD in cardiac muscle was decreased significantly in groups EP and the MDA in cardiac muscle was decreased significantly in group EP.Conclusion:Hongqi formular combined with exercise preconditioning produces cardio-protective effect during myocardial ischemic reperfusion,which may be related with the enhanced anti-oxidant ability,the decreased blood cell damage,the maintainace of vascular endothelium function,the improved microcirculation and the decreased calcium overloading.
