Clinical application of genechip based detection system in diagnosing mycobacterium infection and TB drug resistance gene mutation
10.3969/j.issn.1673-4130.2017.05.025
- VernacularTitle:应用基因芯片检测系统诊断结核分枝杆菌感染及耐药基因突变的临床研究
- Author:
Guohui CHEN
;
Dashan FENG
;
Xiuli WANG
;
Fukang LUO
;
Jun WU
- Keywords:
genechip;
Mycobacterium tuberculosis;
non-tuberculosis mycobacteria;
multidrug resistance;
TB-IFN-γ re-lease assay
- From:
International Journal of Laboratory Medicine
2017;38(5):638-640,643
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical application value of genechip detection system in the mycobacterial species iden-tification and drug resistance analysis .Methods The specimens of sputum ,punctured pus ,pleural and abdominal ascites ,cerebro-spinal fluid and so on were performed the Mycobacterium DNA detection by using the gene chip technique .Then Mycobacterium tuberculosis positive samples were further performed the drug-resistant analysis .Meanwhile the Ziehl-Neelsen acid-fast staining was adopted to detect the sample .The positive rates were compared between the two groups .And TB-IGRA was used to examine the tubercle bacillus infection in partial patients .Results In 4402 samples ,137 cases (3 .36% ) of M ycobacterium tuberculosis (MTB) and 11 cases(7 .4% ) of non-tuberculosis mycobacterium(NTM) were detected .Puncture solution ,bronchoalveolar lavage fluid and tissue specimen had higher positive rate .In the 137 positive M TB samples by rifampicin-resistant gene rpoB ,isoniazide-re-sistant gene katG and inhA detection ,22 cases of resistance gene mutations were detected ;the positive rate of genechip for detecting sputum ,cerebrospinal fluid ,hydrothorax and ascites was higher than that of acid-fast staining .TB-IGRA detection had higher pos-itive rate of TB infection than genechip .Conclusion The genechip detection system can directly conduct Mycobacterium identifica-tion and drug resistance analysis ,which is especially suitable for sputum ,cerebrospinal fluid ,hydrothorax and ascites samples ,and which is simple and rapid with higher sensitivity and good specificity .
