Mechanism of RING finger protein 34 involved in innate immunity:a preliminary study
10.7644/j.issn.1674-9960.2017.01.010
- VernacularTitle:RNF34调控天然免疫的初步研究
- Author:
Yongjie ZHU
;
Pingping ZHANG
;
Pengyu ZHOU
;
Penghao WANG
;
Jiankang CHEN
;
Yinyin TIAN
;
Xiang HE
;
Hui ZHONG
- Keywords:
RNF34;
innate immunity;
RIG-Ⅰ;
NF-κB;
IFN-β
- From:
Military Medical Sciences
2017;41(1):43-47,61
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of RING finger protein 34 ( RNF34 ) involved in innate immunity . Methods Recombinant PCR was used and transient expression of the plasmid was achieved in HEK 293T cells.The cells were stimulated with Sendai virus ( SeV) or N-RIG-Ⅰfor the indicated time while luciferase activity was observed using the dual-luciferase reporter assay kit .Results We constructed the plasmid pcDNA 3-Flag-RNF34 and its three mutations .The study found that when stimulated by SeV , RNF34 could inhibit the activity of NF-κB and IFN-βmore significantly than RNF34-ΔFYVE, RNF34-ΔCID and RNF34-ΔRING.We also found that RNF 34 and its three mutants had similar inhibitory effect when the activation of NF-κB and IFN-βwas stimulated by the N-RIG-Ⅰ.Conclusion RNF34 negatively regulates innate immunity by acting on the RIG-Ⅰ-MAVS signaling pathway .