Mechanism of RING finger protein 34 involved in innate immunity:a preliminary study

VernacularTitle:RNF34调控天然免疫的初步研究
Author: Yongjie ZHU ; Pingping ZHANG ; Pengyu ZHOU ; Penghao WANG ; Jiankang CHEN ; Yinyin TIAN ; Xiang HE ; Hui ZHONG
Keywords: RNF34; innate immunity; RIG-Ⅰ; NF-κB; IFN-β
From: Military Medical Sciences 2017;41(1):43-47,61
CountryChina
Language:Chinese
Abstract: Objective To study the mechanism of RING finger protein 34 ( RNF34 ) involved in innate immunity . Methods Recombinant PCR was used and transient expression of the plasmid was achieved in HEK 293T cells.The cells were stimulated with Sendai virus ( SeV) or N-RIG-Ⅰfor the indicated time while luciferase activity was observed using the dual-luciferase reporter assay kit .Results We constructed the plasmid pcDNA 3-Flag-RNF34 and its three mutations .The study found that when stimulated by SeV , RNF34 could inhibit the activity of NF-κB and IFN-βmore significantly than RNF34-ΔFYVE, RNF34-ΔCID and RNF34-ΔRING.We also found that RNF 34 and its three mutants had similar inhibitory effect when the activation of NF-κB and IFN-βwas stimulated by the N-RIG-Ⅰ.Conclusion RNF34 negatively regulates innate immunity by acting on the RIG-Ⅰ-MAVS signaling pathway .