Preliminary identification of the outer membrane protein H receptors of avian Pasteurella multocida
10.3969/j.issn.1002-2694.2017.01.002
- VernacularTitle:禽多杀性巴氏杆菌外膜蛋白H受体的初步鉴定
- Author:
Wulumuhan NAZIERBIEKE
;
Dong WEI
;
Entomack BORRATHYBAY
;
Di XIAO
- Keywords:
avian Pasteurella multocida;
OmpH;
receptor;
ATP synthase beta subunit
- From:
Chinese Journal of Zoonoses
2017;33(1):9-14,21
- CountryChina
- Language:Chinese
-
Abstract:
To identify the receptors for the outer membrane protein H (OmpH) of avian P.multocida,the membrane proteins of chicken embryo fibroblast (CEF) cells were separated by SDS-PAGE and analyzed by Ligand blot.The OmpH-binding protein was identified by MALDI-TOF mass spectrometry,and its distribution in the membrane proteins of different host esophageal mucosal cells was detected by Ligand blot,ELISA and immunofluorescence microscopy,respectively.Ligand blot analysis showed that a 49-kDa membrane protein of CEF cells bound to recombinant OmpH,and MALDI-TOF spectral results demonstrated that the OmpH-binding protein was ATP synthase β subunit.In addition,the OmpH receptor was present in the chicken and rabbit mucosal cell membranes,but was not detected in the bovine and swine mucosal cell membranes.The above results indicate that the OmpH receptor may be CEF cell-derived ATP synthase beta subunit.
