Value of lymphocyte CD3+ ,CD4+ and CD8+ subsets detection in early diagnosis of neonatal septicemia
10.3969/j.issn.1673-4130.2017.05.016
- VernacularTitle:淋巴细胞CD3+、CD4+、CD8+亚群检测在早发型新生儿败血症诊断中的价值
- Author:
Lihua GAO
- Keywords:
sepsis;
flow cytometry;
lymphocyte;
newborns
- From:
International Journal of Laboratory Medicine
2017;38(5):615-616,619
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the value of T-lymphocyte subsets CD3+ ,CD4+ and CD8+ percentage detection in the diag-nosis of early-onset neonatal sepsis .Methods The neonates born in January 2014 to June 2015 with suspected infection and onset within 7 d were included into this study .The venous blood was collected within 24 h after admission .CD3+ ,CD4+ ,CD8+ lympho-cyte percentage ,C reactive protein and blood routine were detected .The blood culture was performed before the use of antibacterial drugs .The full-term neonates with early onset sepsis confirmed by blood culture were taken as the sepsis group and those of nega-tive blood culture was taken as the local infection group select the blood culture negative for local infection group .At the same time , the hospitalized full-term neonates with hyperbilirubinemia (excluding cases caused by factors such as infection ) served as the con-trol group .The flow cytometery was adopted to detect the samples of above neonatal patients .The lymphocyte subsets CD3+ , CD4+ and CD8+ percentages were observed and compared among 3 groups .Results CD3+ [(40 .3 ± 10 .6)% ] ,CD4+ [(28 .6 ± 11 .2)% ] and CD8+ [(10 .8 ± 2 .6)% ] in the sepsis group were lower than CD3+ [(64 .8 ± 9 .8)% ] ,CD4+ [(48 .9 ± 10 .2)% ] , CD8+ [(17 .6 ± 5 .6)% ] in the local infection group and CD3+ [(62 .6 ± 11 .6)% ] ,CD4+ [(46 .4+13 .6)% ] and CD8+ [(16 .5 ± 7 .3)% ] in the non-infection group ,the differences were statistically significant (P<0 .05);CD3+ ,CD4+ and CD8+ had no statisti-cal difference between the local infection group and non-infection group(P>0 .05) .Conclusion CD3+ ,CD4+ ,CD8+ percentage can be used as an indicator for the diagnosis of early onset neonatal sepsis .
