Simultaneous Determination of Sodium Valproate and Its Metabolite in Human Plasma by RP-HPLC
10.6039/j.issn.1001-0408.2017.05.09
- VernacularTitle:RP-HPLC法同时测定人血浆中丙戊酸钠及其代谢产物的浓度
- Author:
Lingmin ZHANG
;
Juan XIE
;
Longkuan LI
;
Ronghua LIAO
;
Zhongyan QIU
- Keywords:
Sodium valproate;
2-propyl-2-pentenoic acid;
RP-HPLC;
Derivatization;
Plasma concentration
- From:
China Pharmacy
2017;28(5):611-613,614
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of sodium valproate(VPA)and its metabo-lite 2-propyl-2-pentenoic acid (2-ene-VPA) in human plasma. METHODS:Plasma sample was extracted with cyclohexane and experienced derivatization with 2,4′-dibromoacetophenone using n-octanoic acid as an internal standard. RP-HPLC method was adopted. The determination was performed on Zorbax SB-C18 column with mobile phase consisted of acetonitrile-water(65∶35,V/V)at the flow rate of 1 mL/min. The column temperature was set at 35 ℃,and UV dectection wavelenth was set at 258 nm. The sample size was 20 μL. RESULTS:The linear range of VPA and 2-ene-VPA were 5.0-200.0,0.5-20.0 μg/mL(r=0.999 9, n=5). The limits of quantification were 5.0,0.5 μg/mL. RSDs of inter-day and intra-day were all lower than 5%. Method recov-eries were 95.99%-98.80%and 97.40%-98.17%,and extraction recoveries were 80.46%-86.23%and 80.45%-85.61%. The plas-ma concentrations of VPA in 10 epileptic children were 27.4-93.2 μg/mL,and those of 2-ene-VPA were 0.85-3.94 μg/mL,respec-tively. CONCLUSIONS:The method is simple,specific and suitable for plasma concentration determination and pharmacokinet-ic study of VPA.
