Effects of pioglitazone on expressions of TLR4 in peritoneal macrophage foam cell
10.3760/cma.j.issn.1008-6706.2017.06.011
- VernacularTitle:吡格列酮对腹腔巨噬细胞源泡沫细胞形成及 TLR4表达的影响
- Author:
Wei JIANG
;
Shumei WANG
- Keywords:
pioglizaone;
attherosclerosis;
TLR4;
macrophage
- From:
Chinese Journal of Primary Medicine and Pharmacy
2017;24(6):841-843,961
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Pioglitazone on TLR4 expression and peritoneal macrophage foam cells formation induced by ox - LDL.Methods Foam cells were cultured equally divied into four groups in random:the normal control,pioglizaone group,ox-LDL group and ox-LDL +pioglizaone group.The SD rats peritoneal macrophages were pretreated with ox - LDL(50 mg/mL)and Pioglitazone(20 mmo1 /L)respectively for 24 h.Then they were stained by oil red O to determine foam cells formation.The effects of Pioglitazone on foam cells TLR4 ex-pressions were detected by immunohistochemistry.Results The SD rats peritoneal macrophages of control group and Pioglitazone group are not almost stained by oil red O,but the macrophages of ox-LDL group become dark red after stained by oil red O,and the cells color of ox-LDL + Pioglitazone group is thinner than that of ox-LDL group.The expressions of TLR4 in ox-LDL group are higher than those in control and Pioglitazone group significantly(24 h,t =3.015,t =4.007;48 h,t =3.100,t =4.008,P <0.01).while expressions in ox-LDL +Pioglitazone group are lower than ox-LDL group respectively(24 h,t =4.075;48 h,t =4.150,P <0.05).Conclusion Pioglitazone might inhibit the formation of foam cells by suppressing the expressions of TLR4 in macrophages.
