Study on the Anti-endotoxin Active Parts of Lidanpaidu Prescription Based on a Half-in vivo Method
- VernacularTitle:基于半体内法研究利胆排毒方抗内毒素活性部位
- Author:
Fan ZHANG
;
Ruiqin CUI
;
Bisheng HUANG
;
Yan CAO
- Keywords:
Lidanpaidu prescription;
Anti-endotoxin effect;
Active part
- From:
China Pharmacist
2017;20(3):458-459,497
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To further confirm the anti-endotoxin active parts of Lidanpaidu prescription through observing the anti-en-dotoxin intensity based on a half-in vivo method. Methods:The extracting solution of Lidanpaidu prescription was extracted by the sol-vents with different polarity to obtain the active part. The anti-endotoxin activity of the samples was determined by using the Azo color matrix method, and the amount of inactivated endotoxin was compared among the various extraction parts with different dosages. Re-sults:Compared with that of the control group, the amount of inactivated endotoxin by the rat plasma in each administration group was all higher, and the differences were statistically significant (P<0. 05). The rat plasma of the whole prescription administration groups all showed endotoxin inactivation, while the differences among the groups with various dosages were without statistical significance( P>0. 05). The rat plasma of the water soluble fraction administration groups also showed endotoxin inactivation, and the difference be-tween the high dosage group and the low dosage group was statistically significant (P<0. 05). Conclusion:The results of the half-in vivo method showed the consistent anti-endotoxin effect of the whole prescription and the water soluble fraction, furthermore, the dose-effect relationship is preliminarily appeared, suggesting that the water soluble fraction remains the effective components of the whole prescription to a great extent. Therefore, the water soluble fraction can be further confirmed as the active anti-endotoxin part of Lidan-paidu prescription.
