Effects of miR-29s Family on the Biological Behaviors of Glioma Stem Cells

VernacularTitle:miR-29s家族对人脑胶质瘤干细胞生物学行为的影响
Author: Zhuo XI ; Yixue XUE ; Jun MA ; Yunhui LIU
Keywords: glioma stem cells; miR-29a; miR-29b; miR-29c; biological behaviors
From: Journal of China Medical University 2017;46(3):201-204,209
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression of miR-29s in the glioma stem cells,and explore how the members of miR-29s affect the bio-logical behaviors of glioma stem cells. Methods Eight patient specimens were used to culture glioma stem cells. Real-time PCR was adopted to test the expression of miR-29s. CCK-8 analysis was performed to test the proliferation ,Transwell was used to test cell migration and invasion ,and flow-cytometry analysis was carried out to test apoptosis. Results miR-29a,miR-29b and miR-29c were decreased in glioma stem cells. Over-ex-pression of miR-29s could inhibit the proliferation,cell migration and invasion,but promote apoptosis of glioma stem cells. Conclusion miR-29s acts as a cancer suppressor gene in the glioma stem cells ,and miR-29a plays the dominant functional role in the family.