Establishment and Evaluation of Improved Hemisection Model of Spinal Cord Injury in Rats
10.12007/j.issn.0258-4646.2017.03.008
- VernacularTitle:改良脊髓半横断损伤大鼠模型的建立及评价
- Author:
Deri SUN
;
Huiyuan HU
;
Yue ZHU
- Keywords:
spinal cord injury;
rat models;
modification
- From:
Journal of China Medical University
2017;46(3):223-226,237
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an improved hemisection model of spinal cord injury(SCI)in rats with less severe injury and increased sur-vival rate. Methods Female Sprague-Dawley rats(n=20)were randomly divided into 2 groups:standard and improved hemisection model of SCI. Basso-Beattie-Bresnahan(BBB)scores were carried out to determine the hindlimb locomotor function at 1 d,3 d,1 week and 2 weeks post SCI,respectively. In addition,duration of operation,blood loss,location of posterior midline,and success rate of spinal cord exposure during the second surgery were used as the main parameters to evaluate the significance of the new model. Results Compared with the standard hemisection model,the improved hemisection model of SCI exhibited shorter duration of operation,less blood loss,higher BBB score,more accurate location of posterior midline,and higher success rate of spinal cord exposure during the second surgery. Based on the improved hemisection model of SCI, transplantation model of scar tissue in spine cord was then successfully constructed with a success rate above 70%. Conclusion The improved hemisection model of SCI shows significant advantages in many aspects related to the operation,which is applicable to the study of SCI,especially the non-acute SCI.
