Curcumin induce the radiosensitivity of human lung carcinoma NCI-H460 cells through the NF-κB pathway
10.3969/j.issn.1671-8348.2017.06.009
- VernacularTitle:姜黄素通过影响NF-κB信号通路促进肺癌细胞的放射敏感性
- Author:
Xiaobo LI
;
Fang XU
- Keywords:
curcumin;
γ ray;
cell proliferation;
NF-κB;
radiation tolerance
- From:
Chongqing Medicine
2017;46(6):749-751,754
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the combination effectof curcumin and γray on the activity of human lung carcinoma NCI-H460 cells and explore the sensitization of curcumin to γray.Methods The NCI-H460 cells proliferation were detected by MTT,the cell cycle and apoptosis by flow cytometry.The expression of Bcl-2 and Bax were detected by Western Blot and the NF-κB gene expression by RT-PCR.In addition,the mice model of lung cancer was randomly divided into 4 groups:control group,curcumin group,γ ray group and combination group.After 28 days,the tumor volume was measured.Results The proliferation and cell cycle of NCI-H460 cells were inhibited and the apoptosis was increased in combination group.In addition,compared with curcumin group or γray group,the expression of Bcl-2 was inhibited,but the expression of Bax was increased and the mRNA expression of NF-KB was inhibited in combination group(all P＜0.05).Also in combination group the tumor volume was significantly inhibited compared with curcumin group or γ ray group(all P＜0.05).Conclusion Curcumin might induce the radiosensitivity of Human Lung Carcinoma NCI-H460 cells through the pathway.
