Study on HO-1 expression in hunman esophageal carcinoma and correlates with chemotherapy sensitivity of fluorouracil

VernacularTitle:食管癌血红素加氧酶-1表达水平与氟尿嘧啶疗效的关系研究
Author: Jian ZHANG ; Dehua MA ; Baofu CHEN ; Haixie GUO ; Bo ZHANG ; Chengchu ZHU
Keywords: esophageal neoplasms; heme Oxygenase-1; fluorouracil; drug therapy; tolerance
From: Chongqing Medicine 2017;46(6):729-731,734
CountryChina
Language:Chinese
Abstract: Objective To explore the HO-1 expression levels with 5-FU chemosensitivity in esophageal squamous cell carcinoma.Methods Eca109 cells used in all experiments,MTT assay was used in cell growth curve and inhibit rate.RT-PCR and Western blot was used to detect HO-1 in cells treated with different concentrations of ZnppⅨ (0,20,80,100 μmol/L).Results The inhibitory rate of cells was significantly increased when the concentrition of ZnppⅨ increased.The inhibitory rate of cells in 80 μmol/L ZnppⅨ was higher than 20 μmol/L ZnppⅨ group(P＜0.05).The expression of HO-1 mRNA in each group was 0.50± 0.17,0.55±0.15,0.58 ± 0.09 and 0.55 ± 0.16,respectively,there was no significant difference between the two groups (P＞ 0.05).The expression of HO-1 was 0,85±0.07,0.63±0.11,0.43±0.12 and 0.25±0.10,respectively.The expression of HO-1 had significant difference (F=20.01,P＜0.01).Conclusion Eca109 cells inhibition rate positive correlated with ZnppⅨ concentrations,and ZnppⅨ were inhibited the expression of HO-1.not from gene level,but after the translation level.