Partial denture metal framework may harbor potentially pathogenic bacteria.
- Author:
Cristiane Machado MENGATTO
1
;
Leonardo MARCHINI
;
Luciano Angelo de Souza BERNARDES
;
Sabrina Carvalho GOMES
;
Alecsandro Moura SILVA
;
Celia Marisa RIZZATTI-BARBOSA
Author Information
- Publication Type:Original Article
- Keywords: Framework; Denture plaque; Microorganisms; 16S rDNA gene; Removable partial denture
- MeSH: Actinobacteria; Bacteria*; Bacteroidetes; Biofilms; Clone Cells; Databases, Nucleic Acid; Denture, Partial*; Denture, Partial, Removable; DNA, Ribosomal; Female; Follow-Up Studies; Fusobacteria; Humans; Oral Health; Pilot Projects; Polymerase Chain Reaction; Proteobacteria; Trees
- From:The Journal of Advanced Prosthodontics 2015;7(6):468-474
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: The aim of this study was to characterize and compare bacterial diversity on the removable partial denture (RPD) framework over time. MATERIALS AND METHODS: This descriptive pilot study included five women who were rehabilitated with free-end mandibular RPD. The biofilm on T-bar clasps were collected 1 week (t1) and 4 months (t2) after the RPD was inserted (t0). Bacterial 16S rDNA was extracted and PCR amplified. Amplicons were cloned; clones were submitted to cycle sequencing, and sequences were compared with GenBank (98% similarity). RESULTS: A total of 180 sequences with more than 499 bp were obtained. Two phylogenetic trees with 84 (t1) and 96 (t2) clones represented the bacteria biofilm at the RPD. About 93% of the obtained phylotypes fell into 25 known species for t1 and 17 for t2, which were grouped in 5 phyla: Firmicutes (t1=82%; t2=60%), Actinobacteria (t1=5%; t2=10%), Bacteroidetes (t1=2%; t2=6%), Proteobacteria (t1=10%; t2=15%) and Fusobacteria (t1=1%; t2=8%). The libraries also include 3 novel phylotypes for t1 and 11 for t2. Library t2 differs from t1 (P=.004); t1 is a subset of the t2 (P=.052). Periodontal pathogens, such as F. nucleatum, were more prevalent in t2. CONCLUSION: The biofilm composition of the RPD metal clasps changed along time after RPD wearing. The RPD framework may act as a reservoir for potentially pathogenic bacteria and the RPD wearers may benefit from regular follow-up visits and strategies on prosthesis-related oral health instructions.
