Overexpression of Nampt induced cardiac hypertrophy by activating NF-κB
10.3969/j.issn.1001-1978.2017.01.007
- VernacularTitle:过表达Nampt通过激活NF-κB诱导心肌细胞肥大
- Author:
Yi CAI
;
Junjun HUANG
;
Xiawen LIU
;
Biyun HUANG
;
Liu ZHU
;
Bo WU
- Keywords:
Nampt;
cardiac hypertrophy;
NF-κB;
PE;
ANP;
BNP
- From:
Chinese Pharmacological Bulletin
2017;33(1):33-38,39
- CountryChina
- Language:Chinese
-
Abstract:
Aim To examine the effect of Nampt over-expression on cardiac hypertrophy,and elucidate the role of NF-κB.Methods The cultured neonatal car-diomyocytes were pretreated with 100 μmol · L-1 PE or transfected with Nampt.The mRNA and protein ex-pression of Nampt were determined by Real-time PCR and Western blot respectively.The cardiomyocyte hy-pertrophy was monitored by measuring cell-surface area and the mRNA levels of ANP and BNP,which were biomarkers of hypertrophic response.Moreover,we te-sted the effects of Nampt on NF-κB-dependent tran-scription activity through luciferase reporter gene as-says.Results Nampt overexpression significantly in-creased cardiomyocyte surface area and the mRNA ex-pression of ANP and BNP.In addition,Nampt overex-pression could markedly increase NF-κB-dependent transcription activity. Moreover, when p65 was knocked down,cardiomyocytes with Nampt overexpres-sion could not induce cardiac hypertrophy.Conclusion
Overexpression of Nampt induces cardiac hypertro-phy by increasing NF-κB-dependent transcription activ-ity.
