Optimization of a forensic DNA extraction kits and its applicability evaluation

VernacularTitle:法医学DNA提取试剂盒优化及适用性评价
Author: Yiren YAO ; Xue BAI ; Jimin XU ; Youzheng WANG ; Yongmin YU ; Sisi ZHANG ; Xingchun ZHAO
Keywords: forensic biological evidence; DNA extraction; magnetic beads; STR
From: Chinese Journal of Forensic Medicine 2016;31(6):595-597,598
CountryChina
Language:Chinese
Abstract: Objective To construct a rapid genetic DNA extraction method, with nano magnetic beads, self-designed reagents system and extracting process. Method Part I: DNA extraction from old blood cotton swab sample with self-designed DNA extraction kit, then quantiifed by UV spectrophotometer. The method was further optimized on the preliminary results. Part II: All kinds of difficult DNA sample were tested with optimized kit, to detect the applicability of the kit. Result By improving the experimental condition, the extraction effects of different DNA sample is good, meanwhile, the extraction cost is relatively low.