Synthesize of water-solubility derivant of 6,7,4′-trihydroxyisoflavone (T2)and study of its inhibition effect on human breast cancer cell proliferation
10.3969/j.issn.1671-8348.2017.01.005
- VernacularTitle:6，7，4′-三羟基异黄酮（T2）水溶性衍生物的合成及其对宫颈癌细胞抑制作用的研究
- Author:
Shaolong YUAN
;
Rong LI
- Keywords:
isoflavones;
uterine cervical neoplasms;
derivant of trihydroxyisoflavone;
water-solubility;
sulfonation reaction;
anti-tumor
- From:
Chongqing Medicine
2017;46(1):33-35,39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To synthesize of water-solubility derivant of 6,7,4′- trihydroxyisoflavone(T2 ),to characterize its structure and to evaluate its anti-tumor activity.Methods The sulfonic group(-SO3 H)was grafted to 3'position in loop B of T2 through sulfonation reaction,then strong aqua was added to above production,and obtained a water-solubility elevated compound named T2-SO3 (NH4 )2 through ammoniation.The construction of T2-SO3 (NH4 )2 was characterized by 1 H-NMR,13 C NMR,MS and element analysis.Its activity for killing human breast cancer cells (Hela)was analyzed by CCK-8 assay and flow cytometry.Re-sults The two kinds of water-solubility derivant of T2-SO3 H·2H2 O and T2-SO3 (NH4 )2 were obtained through above methods, and their yield rates were 96% and 75% respectively.The biological experiments showed that the anti-tumor activity of T2-SO3 (NH4 )2 was significantly enhanced.Conclusion Compared to T2,T2-SO3 (NH4 )2 exhibit higher biocompatibility and anti-tumor activity with vast biological application prospect.
