Echinococcus granulosus stimulates high PPARα/γ expression and drives polarization of macrophages in vitro
10.3969/j.issn.1000-484X.2017.01.003
- VernacularTitle:原头蚴体外刺激巨噬细胞高表达PPARα/γ并调节其极化
- Author:
Congzhe CHEN
;
Dan DONG
;
Hairui FANG
;
Hongqun JIANG
;
Jun HOU
;
Kun YANG
;
Feng GUO
;
Xueling CHEN
- Keywords:
Echinococcus granulosus;
Macrophage;
PPAR-γ/α;
Polarization
- From:
Chinese Journal of Immunology
2017;33(1):16-19,24
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression levels of PPARα/γin RAW264. 7 cells in the early stages of co-cultivation with Echinococcus granulosus in vitro. Methods:RAW264. 7 cells were co-cultured with E. granulosus and collected at 12,24,36,48, 72 h. The mRNA levels of PPAR-γ,PPAR-α,M1 macrophages-associated cytokines including TNF-α,MCP-1 and IL-1β,and M2 mac-rophages-associated cytokines including Arg-1,TGF-β and Fizz-1 were detected by qRT-PCR. The protein levels of Arg-1 and MR were analyzed by ELISA. Results:The expression levels of PPAR-γ, PPAR-αand the M2 macrophages-associated cytokines including Arg-1,TGF-β,Fizz-1 and MR were significantly increased,especially at 72 h (P<0. 05). M1 macrophages-associated cytokines including TNF-α,MCP-1 and IL-1β were decreased at 72h although increased at first. Conclusion:During the early stages of co-cultivation with Echinococcus granulosus in vitro, the levels of PPAR-γ/α are up-regulated in RAW264. 7 cells, which may drive macrophage polarization and play a role in the immune escape.
