MicroRNA-155 induced invasion and migration of human trophoblast cells via CXCR4/PI3 K/AKT signaling pathway
10.3969/j.issn.1000-484X.2017.01.008
- VernacularTitle:MicroRNA-155通过调节CXCR4/PI3 K/AKT途径影响滋养细胞的侵袭与迁移
- Author:
Zhan ZHANG
;
Aiping LI
;
Yuanyuan WANG
;
Wanyu SONG
;
Na XU
;
Hui LIU
;
Jie ZHOU
- Keywords:
miR-155;
CXCR4;
PI3K/AKT;
Trophoblast cells;
Preeclampsia
- From:
Chinese Journal of Immunology
2017;33(1):41-46,51
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect on the CXCR4/PI3K/AKT pathway after the transfection of miR-155 mimics and miR-155 inhibitor combined with the research on the ability of invasion and migration of human chorionic JEG-3 trophoblast cells. Methods:Chemically synthesized miR-155 mimics and miR-155 inhibitor were transfected into JEG-3 cells. The effect on the ability of invasion and migration were analyzed by Transwell migration assay and Wound healing assay. The expression of CXCR4 mRNA was detected by Real-time PCR. The expression of CXCR4 and p-AKT protein were detected by Western blot. Results: Transfection with miR-155 mimics significantly down-regulated the expression of CXCR4 as compared with the control group(P<0. 05);JEG-3 cells transfected miR-155 mimics had lower levels of migration and invasion capacity than cells in the control group(P<0. 05). However, transfection with miR-155 inhibitor significantly up-regulated the expression of CXCR4 as compared with the control group(P<0. 05);JEG-3 cells transfected miR-155 inhibitor had higher levels of migration and invasion capacity than cells in the control group ( P<0.05).Addition,the expression of p-AKT protein of JEG-3 cells was down-regulated after transfected miR-155 mimics,and the expression of p-AKT protein of JEG-3 cells was up-regulated after transfected miR-155 inhibitor. Conclusion:miR-155 may inhibits the invasion and migration of trophoblast cells by regulating CXCR4/PI3K/AKT pathway contributing to the development of preeclampsia.
