Experimental research of miR-132 inhibits proliferation and induces apoptosis of ovarian cancer via Ezrin
10.3969/j.issn.1000-484X.2017.01.014
- VernacularTitle:miR-132靶向调控Ezrin抑制卵巢癌细胞增殖、促进凋亡的实验研究
- Author:
Bo YANG
;
Shengze LI
;
Ling MA
;
Suyang GUO
;
Hongli LIU
;
Jian LIU
;
Junjun SHAO
- Keywords:
miR-132;
Ovarian cancer;
Proliferation;
Apoptosis;
Ezrin
- From:
Chinese Journal of Immunology
2017;33(1):72-75,80
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the biological function of miR-132 in ovarian cancer and the target. Methods: 22 cases ovarian cancer tissue and non-tumor tissue adjacent were collected,the expression of miR-132 in tumor tissue and non-tumor tissue, normal ovarian epithelial cells and ovarian cancer cell were detected by RT-PCR. The normal ovarian epithelial cells which the expression of miR-132 maximum or minimum were chosen, and they were divided into two groups, respectively with transfection of negative control plasmid ( NC) and miR-132 mimic plasmid. The expression of miR-132 after transfection was detected by RT-PCR,the cell proliferation and cell apoptosis were detected by CCK-8 method and flow cytometry instrument respectively,the expression of Ezrin protein was detected by Western blot. Results:The expression of miR-132 in tumor tissue was significantly lower than the tumor tissue adjacent,the expression of miR-132 in ovarian cancer cell lines was significantly lower than normal ovarian epithelial cells, the differences were statistically significant (P<0. 05). The SKOV3 cell lines was chosed for gene transfection,compared with NC group, transfection with miR-132 mimic plasmid could significantly reduce cell proliferation, increase cell apoptosis, the difference had statistical significance ( P<0. 05 ) . Western blot results showed that up-regulation miR-132 significantly increased the Ezrin protein expression in ovarian cancer SKOV3 cells ( P<0. 05 ) . Conclusion: In ovarian cancer, miR-132;inhibits proliferation and induces apoptosis of ovarian cancer via Ezrin,it may be a tumor suppressor gene.
