Related research of immunosuppression in intermingled skin transplantation induced by B7-H1 expressed on auto-keratinocytes
10.3969/j.issn.1000-484X.2017.02.003
- VernacularTitle:混合皮肤移植中自体角朊细胞表达B7-H1诱导免疫抑制的相关研究
- Author:
Weidong CAI
;
Ying BAI
;
Xianjin ZHU
;
Peizheng ZHENG
;
Yingping CAO
;
Guangyan ZHOU
- Keywords:
Keratinocytes;
B7-H1;
Intermingled skin transplantation;
Immunosuppression
- From:
Chinese Journal of Immunology
2017;33(2):174-177,185
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role and mechanism of the B7-H1 expressed by auto-keratinocytes in the intermingled skin grafting model in vitro(MELC). Methods:The intermingled skin grafting model(MELC) was established in vitro. Flow cytometry was performed to detect the expressions of B7-H1 in keratinocytes. The expressions of PD-1 in lymphocytes were measured at the same time. The levels of IL-10,Foxp3 and GATA-3 mRNA in lymphocytes were detected by Real-time quantitative PCR. Results: Through flow eytometry,in the MELC with auto-keratinocytes,the expression of B7-H1 in auto-keratinocytes and the PD-1 in lymphocytes were rising trend and the rising rate was in time-dependent manners(P<0. 01). RT-PCR assay indicated that the relative levels of IL-10, Foxp3,GATA-3mRNA expression were significant raised and the rising rate was in time-dependent manners (P<0. 01). Conclusion:In the intermingled skin grafting model,the auto-keratinocytes could express B7-H1 to enhance the expression of PD-1 in T cells. When B7-H1 combined with PD-1,the Th2 cells and Foxp3+Tregs were induced and suppressed the immune response in the intermingled skin grafting model.
