Expression and clinical relevance of IRAK-M in monocytes in patients with systemic lupus erythematosus
10.3969/j.issn.1000-484X.2017.02.019
- VernacularTitle:白介素-1受体相关激酶-M在系统性红斑狼疮患者单核细胞中的表达及与临床的相关性
- Author:
Tongping HU
;
Li BAI
;
Wenlan ZHANG
- Keywords:
SLE;
IRAK-M;
SLEDAI
- From:
Chinese Journal of Immunology
2017;33(2):256-258,263
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression and clinical relevance of IRAK-M in monocytes in patients with systemic lupus erythematosus. Methods: Real-time quantitative PCR was performed for IRAK-M mRNA measurement and enzyme-linked immunosorbent assay was used for anti-double-stranded DNA antibody ( dsDNA ) and anti-single-stranded DNA antibody ( ssDNA ) . Dynamic scattering turbidimetric immunoassay was applied for complement 3(C3),complement 4(C4) and C-reactive protein(CRP), while Westergren method for erythrocyte sedimentation rate (ESR). Correlation analysis of IRAK-M with SLEDAI,dsDNA,ssDNA,C3, C4,CRP and ESR was computed by Pearson or Spearman. Results:①The result showed that the mRNA expression of IRAK-M in SLE patients was significantly lower than healthy controls (P<0. 05);and the mRNA expression of IRAK-M in active group was significantly lower than stable group ( P<0. 05 ) . ②The levels of dsDNA, ssDNA, CRP and ESR in SLE patients were significantly higher than healthy controls( P<0. 05 );and the levels of C3 and C4 in SLE patients were significantly lower than healthy controls ( P<0. 05 ) . Meanwhile,the levels of dsDNA,ssDNA,CRP and ESR in active group were significantly higher than stable group (P<0. 05),and the levels of C3 and C4 in active group were significantly lower than stable group (P<0. 05).③Correlation analysis showed that the mRNA expression of IRAK-M had a positive correlation with C3 (P<0. 05),and had a negative correlation with SLEDAI,dsDNA,CRP and ESR (P<0. 05),but had no correlation with ssDNA and C4(P>0. 05). Conclusion: This study indicated that IRAK-M play certain significant roles in the pathogenesis of SLE. We can monitor SLE disease activity and prognosis by quantitative detection of mRNA expression of IRAK-M. Meanwhile,it is very necessary to routinely test and regularly monitor the levels of dsDNA,ssDNA,C3,C4,CRP and ESR in SLE.
