A murine model of Th2 response induced by shrimp tropomyosin
10.3969/j.issn.1000-484X.2017.02.014
- VernacularTitle:一种以虾原肌球蛋白为致敏原的Th2反应小鼠模型的建立
- Author:
Lei FANG
;
Rui HOU
;
Qiaoling FEI
;
Yuan GAO
;
Runlan CAI
;
Yun QI
- Keywords:
Th2 response;
Shrimp tropomyosin;
Basophil;
IgE
- From:
Chinese Journal of Immunology
2017;33(2):233-236,241
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop murine models of Th2 response induced by shrimp tropomyosin (ST). Methods:Mice were sensitized with ST for 6 weeks. The serum antigen-special IgE (sIgE),total IgE and sIgG level,Th1/Th2 cytokines production were measured by ELISA. The basophil activation in mice was measured by flow cytometry. Results:The intraperitoneal sensitization with ST for 6 weeks induced significant increase of serum sIgE,total IgE and sIgG (sIgG1,sIgG2a and sIgG2b) level in mice. Th2 cell response was induced and cytokines (IL-4,IL-5,IL-10 and IL-13) production increased in splenocytes stimulated by ST,while Th1 cytokine (IFN-γ) production decreased. As the markers of basophil activation,CD200R and CD41 expression also increased in response to ST. Conclusion:The Th2 response is dominant in ST-induced anaphylaxis in mice.
