Study on the aminoglycoside resistance gene of nosocomial multidrug-resistant Acinetobacter baumannii
10.3969/j.issn.1673-4130.2017.02.003
- VernacularTitle:多重耐药鲍曼不动杆菌氨基糖苷类耐药基因的研究
- Author:
Yunhua MA
;
Zhijun ZHANG
;
Fen SU
;
Haiyan WU
- Keywords:
multidrug-resistant;
Acinetobacter baumannii;
aminoglycoside resistance gene;
distribution of specimens
- From:
International Journal of Laboratory Medicine
2017;38(2):151-152,156
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the prevalence of aminoglycoside resistance gene in multidrug-resistant Acinetobacter bau-mannii isolated in clinical at a certain time,and to provide the basis for the control of nosocomial infection.Methods 9 strains of multidrug-resistant Acinetobacter baumannii were isolated in First People′Hospital of Weifang from November 26,2013 to Decem-ber 12,2013.Identification of bacteria and susceptibility testing were conducted by VITEK2,and partial antimicrobial drug suscepti-bility tests were performed by the disk diffusion method.Aminoglycoside resistance genes were detected by PCR and the positive genes were partly sequenced.Results Among the 9 stains of multidrug-resistant Acinetobacter baumannii,2 strains carried aac(3)-Ⅰ gene,3 carried ant(3″)-Ⅰ gene,3 carried aac(6′)-Ⅰ gene.AndarmA gene was positive in 9 strains.All strains were resistant to aminoglycosides,such as amikacin,gentamicin and tobramycin.There were 5 specien issolated in ICU,while 3 specimens were isola-ted in neurosurgery ward.All specimens were separated from sputum.Conclusion Antimicrobial resistance to aminoglycosides of Acinetobacter baumannii isolated in the hospital during this time was related to aminoglycoside resistance gene.Nosocomial infec-tion caused by multidrug-resistant Acinetobacter baumanniiin,ICU and neurosurgery ward should be vigorously monitored.
