Effect of Oxymatrine Ointment on Mice with Chronic Eczema
- VernacularTitle:氧化苦参碱软膏对小鼠慢性湿疹的影响
- Author:
Lihua ZHANG
;
Yan CHEN
;
Xuemei FANG
;
Shuting ZHENG
- Keywords:
Oxymatrine ointment;
Chronic eczema;
Inflammatory cell count;
Mice;
Efficacy;
Mechanism
- From:
China Pharmacist
2017;20(1):46-48,49
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of oxymatrine ointment on chronic eczema in mice, and preliminarily explore the mechanism. Methods:Thirty-two Kunming mice were randomly divided into four groups including oxymatrine ointment group, blank model group, blank ointment group and positive drug group treated with compound dexamethasone acetate cream. Eczema skin was con-tinuously treated with drugs for 14 days. The mice were sacrificed on the 15th day, and the eczema skin was clipped from back to make histological sections. The changes of inflammation were observed, and the inflammatory cell count was obtained. Meanwhile, heart blood was collected, and serum was obtained by centrifugation. The serum levels of IL-4 and IL-1β were determined by ELISA. Re-sults:The inflammatory cell count in oxymatrine ointment group and the positive drug group was lower than that in the blank model group and the blank ointment group (P<0. 05). The pathology results showed that oxymatrine ointment could improve chronic eczema symptoms, reduce the inflammatory cell infiltration and decrease edema, which was similar to the effects of compound dexamethasone acetate cream. In the respect of molecular immunology, the IL-4 and IL-1βlevels in serum showed no significant changes in oxymatrine ointment group (P>0. 05). Conclusion: Oxymatrine ointment has certain anti-inflammatory effect on eczema, and the mechanism needs to be studied further.
