Optimization of Flash Extraction for Isatidis Radix by Box-Behnken Experimental Design
- VernacularTitle:Box-Behnken效应面法优化板蓝根药材闪式提取工艺
- Author:
Baohong YAN
;
Shimin YANG
- Keywords:
Isatidis Radix;
Epigoitrin;
Flash extraction;
Box-Behnken experimental design
- From:
China Pharmacist
2017;20(1):64-67,68
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the flash extraction process of Isatidis Radix by Box-Behnken experimental design. Methods:A flash extraction method was used with the extraction voltage, material-liquid ratio and extraction time as the independent variables. A Hassan’s method was used to calculate the normalized value(OD) of comprehensive evaluation of the dry extract yield and the epi-goitrin extraction rate in order to establish the mathematical relationship between the comprehensive evaluation OD and the independent variables, and the response surface method was used to predict the best process conditions. Results:The optimum process conditions were as follows:voltage of 88 V, material-liquid ratio of 19. 62, and extraction time of 2. 03 min. The rate of dry extract and epigoitrin extraction was 37. 902% and 0. 1887%, respectively. Conclusion:The measured value is close to the predicted one,which indicates the comprehensive flash extraction parameters optimized by Box-Behnken experimental design can be used for the extraction for Isatidis Radix.
