Determination of Nitroglycerin Ointment by HPLC
- VernacularTitle:HPLC法测定硝酸甘油软膏的含量
- Author:
Guixiang ZHONG
;
Jia YAN
;
Xin ZHOU
;
Aiwen HUANG
;
Hongtao SONG
- Keywords:
Nitroglycerin;
Ointment;
Content determination;
HPLC
- From:
China Pharmacist
2017;20(1):182-183,184
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize and improve the content determination method for nitroglycerin ointment. Methods:An HPLC method was used,the column was Hypersil ODS(150 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile ∶water(50 ∶50),the detection wavelength was set at 220 nm,the flow rate was 1 ml·min-1 ,the column temperature was 30℃,and the injection volume was 20 μl. Results: The results showed a good linear relationship within the concentration range of 0. 020 3-0. 203 3 mg · ml-1 ( r =0. 999 9),and the average recovery was 99. 51%(RSD=1. 06%,n=9). Conclusion: The method is rapid,accurate and reproduci-ble,and can be used to determine the content of nitroglycerin in nitroglycerin ointment.
