Study on Preparation of Anti-tumor Drug H6 Polymeric Micelles and Their in vitro Anti-tumor Effects
10.6039/j.issn.1001-0408.2017.04.28
- VernacularTitle:抗肿瘤药H6聚合物胶束的制备及体外抗肿瘤作用研究
- Author:
Chao PAN
;
Huili LIU
;
Junpeng XU
;
Qiaoxin TANG
;
Li WAN
- Keywords:
Anti-tumor drug H6;
mPEG2000-PCL4000;
Micelles;
Anti-tumor effect
- From:
China Pharmacy
2017;28(4):533-535,536
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To prepare anti-tumor drug H6(lactone compound)polymeric micelles,and to investigate its in vitro anti-tumor effects. METHODS:Using mPEG2000-PCL4000 as carrier,H6/mPEG2000-PCL4000 micelles were prepared. Using particle size, PDI and 48 h whether to produce precipitation as indexes,feeding ratio,H6 concentration,volume ratio of organic solvent were screened. The encapsulation efficiency and drug-loading amount of micelle were all detected. MTT assay was used to detect the tox-icity of micelles and H6 solution to human non-small cell lung cancer cell A549 and human lung cancer cell H460. RESULTS:The screened formulation was as follows as feeding ratio of 1∶25,H6 concentration of 2 mg/mL,the ratio of ethanol to chloroform of 1∶1(V/V). The parameters of prepared H6/mPEG2000-PCL4000 micelles were as follows as particle size of(40.74±0.116 3)nm,PDI of(0.101±0.006),encapsulation efficiency of(94.87±0.016 3)%,drug-loading amount of(7.07±0.001 5)%(n=3). IC50 of mi-celles and H6 solution to A549 cell were 15.62 and 12.57 nmol/L;IC50 of micelles and H6 solution to H460 cell were 27.68 and 15.19 nmol/L. CONCLUSIONS:H6/mPEG2000-PCL4000 micelles are prepared successfully and show in vitro anti-tumor effects.
