Comparison of PCR and HRM for detecting bacterial drug resistance gene aac (6′)- Ib-cr
10.3969/j.issn.1673-4130.2017.03.011
- VernacularTitle:PCR和HRM两种方法检测细菌耐药基因aac（6′）-Ib-cr的比较研究
- Author:
Lixia BAI
;
Hong YANG
- Keywords:
high-resolution melt;
gene;
polymerase chain reaction;
bacteria
- From:
International Journal of Laboratory Medicine
2017;38(3):319-320,323
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the difference of detection rate between PCR and high-resolution melt (HRM)for detecting the bacterial drug resistance gene aac(6′)-Ib-cr.Methods The PCR method was adopted to detect the aac(6′)-Ib gene in 299 strains of common Gram-negative bacilli,and the PCR positive products of aac (6′)-Ib were digested with BtsCI.Meanwhile Aac (6′)-Ib and aac (6′)-Ib-cr genes in above bacterial strains were detected by adopting the HRM technique.Results A total of 29 isolates were i-dentified as aac (6′)-Ib gene,and 21 mutations were aac (6′)-Ib-cr,with a mutation rate of 72.4% (21/29)for PCR and restriction enzyme digestion.And the results of the HRM test were in good agreement with each other.Conclusion PCR and HRM are similar for the detection of the bacterial resistance gene aac (6′)-Ib-cr,and aac (6′)-Ib-cr gene could be detected by a simpler HRM tech-nique instead of PCR and enzyme digestion,especially for a large number of screening.
