miR-15a-5p inhibits proliferation and migration of human hepatocellular carcinoma SMMC-7721 cells
10.3969/j.issn.1000-4718.2017.02.024
- VernacularTitle:miR-15a-5p 抑制人肝癌 SMMC-7721细胞增殖与迁移
- Author:
Jiansheng QIAN
;
Yu LI
;
Jianwei DOU
- Keywords:
miR-15a-5p;
Hepatocellular carcinoma;
Cell proliferation;
Cell migration
- From:
Chinese Journal of Pathophysiology
2017;33(2):344-348,352
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the influence of high expression of miR-15a-5p on the proliferation and migration of human hepatocellular carcinoma SMMC-7721 cells.METHODS: The miR-15a-5p oligonucleotide , which was recon-structed with additional restriction sites of EcoRⅠand HindⅢ, was chemically synthesized and confirmed by sequencing . The miR-15a-5p eukaryotic expression system was constructed by pcDNA 6.2-GW/Em-GFP-pre-miR-15a-5p plasmid.The miR-15a-5p was transfected into the SMMC-7721 cells transiently by plasmid , and quantified by quantitative real-time PCR at the mRNA level.The cell viability was measured by CCK-8 assay, and the living cell counting was performed by the method of Trypan blue exclusion .The migration ability of the SMMC-7721 cells with high expression of miR-15a-5p was de-tected by wound healing test .RESULTS: The sequence of miR-15a-5p oligonucleotide 100% matched the designed se-quence.Compared with control group , the miR-15a-5p expression was increased significantly (P<0.05).The viability, the living cell number and the migration ability of the SMMC-7721 cells were decreased in high expression of miR-15a-5p group with statistically significant difference (P<0.05).CONCLUSION: The abilities of proliferation and migration in human hepatocellular carcinoma SMMC-7721 cells are decreased by high expression of miR-15a-5p.
