Artesunate supresses NF-κB and MCP-1 expressions in high glucose-induced rat mesangial cell proliferation model
10.11958/20160973
- VernacularTitle:青蒿琥酯可抑制高糖诱导的肾小球系膜细胞增殖模型中NF-κB、MCP-1的表达
- Author:
Xuejuan HUANG
;
Ke SU
;
Yan LONG
;
Jingqiong XUN
;
Haixu WANG
- Keywords:
diabetic nephropathies;
apoptosis;
mesangial cells;
NF-kappa B;
monocyte chemotactic protein 1;
Artesunate
- From:
Tianjin Medical Journal
2017;45(2):164-167,108
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of artesunate (Art) on cell proliferation, apoptosis, nuclear factor (NF)-κB and monocyte chemotactic protein 1 (MCP-1) expressions induced by high glucose in rat renal mesangial cells (HBZY-1), and the mechanism thereof. Methods HBZY-1 cells were cultured and divided into normal glucose group (5.6 mmol/L), high glucose group (25 mmol/L) and high glucose with different concentrations of Art (10 mg/L, 20 mg/L, 30 mg/L) groups. MTT assay was used to detect the cell proliferation after 48 h. The apoptotic rate was evaluated by flow cytometry with Annexin V-FITC/PI double stains. The protein levels of NF-κB and MCP-1 in the cell culture supernatant were determined using ELISA. Results High glucose induced apoptosis and proliferation in HBZY-1 cells, and the expressions of NF-κB and MCP-1 in the supernatant were also increased (P<0.05). After treatment with Art, the proliferation was obviously abolished, and the apoptosis was increased, and the expressions of NF-κB and MCP-1 in the supernatant were decreased in HBZY-1 cells. The effects of Art showed a dose-dependent manner (P<0.05). Conclusion Artesunate treatment can reverse the effect of high glucose in HBZY-1 cells in a dose-dependent manner, which may provide a new therapeutic strategy for diabetic nephropathy.
