High salt diet enhances the physical coupling between TRPV4 and cPLA2
10.3969/j.issn.1001-1978.2016.12.016
- VernacularTitle:高盐饮食增强内皮细胞TRPV4与cPLA2的空间偶联作用
- Author:
Chunyuan SUN
;
Mingxu XIE
;
Yuying LIU
;
Yanfei CAI
;
Peng ZHANG
;
Jian JIN
;
Xin MA
- Keywords:
TRPV4;
cPLA2;
high salt;
thoracic aorta;
immune-fluorescence resonance energy transfer;
spatial proximity
- From:
Chinese Pharmacological Bulletin
2016;32(12):1718-1722,1723
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the physical coupling between transient receptor potential channel vanilloid type 4 (TRPV4 ) and cPLA2 in endothelial cells. Methods We investigated the physical association of TRPV4-cPLA2 coupling by immunofluorescence reso-nance energy transfer (immuno-FRET)to assess the spatial proximity between TRPV4 and cPLA2 in human microvascular endothelial cells (HMEC),primary cul-tured endothelial cells and in thoracic aortas rings from high salt-induced hypertension mice.Results At the cellular level,with high salt treatment,the physical in-teraction of TRPV4 and cPLA2 was significantly en-hanced in primary vascular endothelial cells and HMEC.Furthermore, in thoracic aortas rings from high salt-induced hypertension mice,we found an in-creases interaction between TRPV4 and cPLA2 in en-dothelial cells from arterial segments .Conclusion High-salt treatment increases the endothelial TRPV4-cPLA2 coupling,indicating that this coupling may pro-vide a new target for vascular endothelial dysfunction.
