Effect of combination Oxymatrine with 5-FU inhibited proliferation in SGC-7901 human gastric cancer cells and its mechanism
10.3969/j.issn.1000-484X.2016.12.012
- VernacularTitle:氧化苦参碱联合5-FU对人胃癌 SGC-7901细胞生长的协同抑制作用及其机制研究
- Author:
Yang LIU
;
Nian LIU
;
Wen LIU
- Keywords:
Oxymatrine;
5-FU;
Gastric cancer;
Proliferation;
Apoptosis;
VEGF
- From:
Chinese Journal of Immunology
2016;32(12):1781-1784,1789
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the synergy inhibition effects of Oxymatrine combine with 5-FU on proliferation and apoptosis of SGC7901 human gastric cancer cells and its mechanism. Methods:SGC7901 cells were treated by different concentrations of Oxymatrine with or without 10. 0 mg/L 5-FU for 24,48 and 72 h. The cells proliferative vitality was detected by MTT assay,and the ap-optosis of SGC7901 cells was detected by HOECHST stain. Immunohistochemistry was applied to examine the protein expression of VEGF. The transcriptions of VEGF mRNA were distinguished by RT-PCR technique. Results:Compared with control group,middle dose and high dose concentration of Oxymatrine groups,and groups of its combined with 5-FU can inhibit the proliferation and induced the apoptosis of SGC7901 cancer cells. Compared with 5-FU group, the inhibition rate and apoptosis rate were significantly increased in groups of Oxymatrine combined with 5-FU. The mRNA and protein expression of VEGF were significantly decreased in groups of Oxymatrine and it combined with 5-FU. Conclusion:Synergy inhibition effects of Oxymatrine combine with 5-FU on proliferation and apoptosis of SGC7901 human gastric cancer cells were found,which may be related to strengthen the effects of Oxymatrine of inhibiting angiogenesis relate with down-regulating the expression of VEGF.
