Association between ERCC2/XPD Polymorphisms and UVC-induced DNA Damage Using Transfected Cells Model

VernacularTitle:应用转染细胞研究ERCC2/XPD基因多态与短波紫外线所致DNA损伤修复的关联
Author: Yangyang GUAN ; Mingyang XIAO ; Liang PAN ; Ping XUE ; Guopei ZHANG ; Xiaobo LU
Keywords: ERCC2/XPD; single nucleotide polymorphisms; transfected cell model; UVC; nucleotide excision repair
From: Journal of China Medical University 2016;45(12):1066-1071,1076
CountryChina
Language:Chinese
Abstract: Objective To explore the function of ERCC2/XPD polymorphisms in the repair of DNA damage induced by UVC. Methods Plas?mids stably expressing ERCC2/XPD rs13181 AA(Lys751)and ERCC2/XPD rs13181 CC(Gln751)were transfected into Chinese hamster ovary cells,and the stable ERCC2 transfected cell lines were obtained. MTT assay was used to compare the inhibitory rates of the transfected cells treated with UVC at different irradiation intensity. The DNA damage repair ability of the transfected cells treated with UVC for 1,3,6 and 24 h was detected by modified comet assay. Results Compared with UV5ERCC2(CC),UV5ERCC2(CC) was more sensitive to UVC with decreased cell viability. DNA damage level of UV5ERCC2(CC) cells was more serious than UV5ERCC2(CC). Conclusion DNA repair capacity of ERCC2/XPD rs13181A allelic is lower than its wild?type,suggesting that ERCC2/XPDpolymorphisms play a critical role in UVC?induced DNA damage repair.