Effects of Lipoic Acid on Bone Metabolism in Osteoporosis Rat and Its Mechanism
10.12007/j.issn.0258-4646.2016.12.017
- VernacularTitle:硫辛酸对骨质疏松大鼠骨代谢的影响及机制研究
- Author:
Lesheng JIA
;
Gang ZHENG
;
Fan XIA
;
Shuang YANG
- Keywords:
osteoporosis;
lipoic acid;
oxidative stress;
osteoprotegerin;
receptor activator of nuclear factor-kB ligand
- From:
Journal of China Medical University
2016;45(12):1133-1135,1138
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of oxidative stress and lipoic acid(antioxidant)on bone metabolism and explore the underlying mechanism. Methods A total of 24 Wistar rats aged 8 weeks were randomly divided into three groups. Osteoporosis rats model was established by bilateral ovaries deleted. Rat in lipoic acid group was injected with lipoic acid(60 mg/kg)for 8 weeks. The bone mineral density(BMD),steo?calcin,ALP,Ca,P,MDA,SOD and GSH?Px were detected. The levels of OPG and RANKL in serum were measured by Western blotting. OPG and RANKL mRNA were detected by real?time PCR. Results The level of BMD level in blood,SOD,GSH?Px,OPG mRNA and protein level in femur of osteoporosis group were significantly lower than the control group(P<0.05). On the other hand,steocalcin,ALP,MDA,RANKL mRNA and protein level were significantly higher than the control group(P<0.05). The level of BMD level in blood,SOD,GSH?Px,OPG mRNA and protein level of lipoic acid group were significantly higher than the osteoporosis group(P<0.05). The steocalcin,ALP,MDA,RANKL mRNA and protein level were significantly lower than the osteoporosis group(P<0.05). Conclusion Oxidative stress may increase osteoporosis through the upregulation of OPG/RANKL pathway in rats ,and antioxidant lipoic acid can alleviate the progress of osteoporosis.
