Detection of Th17, Treg cells and IL-17, IL-23 levels in children with Henoch-Schonlein purpura
10.3969/j.issn.1000-484X.2016.12.017
- VernacularTitle:过敏性紫癜患儿Th17、Treg细胞及IL-17、IL-23水平的测定
- Author:
Tingting ZHANG
;
Xiaofei JI
;
Shaoyou LI
;
Bo ZHAO
;
Jiang DUAN
;
Jingjing XIONG
;
Mei LIU
;
Yongkun HUANG
- Keywords:
Henoch-Schonlein Purpura;
Children;
Th17 cell;
Regulatory T cell;
Interleukin-17;
Interleukin-23
- From:
Chinese Journal of Immunology
2016;32(12):1801-1804,1814
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the changes of Th17,regulatory T(Treg) cells and IL-17,IL-23 levels at acute phase and recovery phase in children with Henoch-Schonlein purpura(HSP) in order to further understand the immunological pathogenesis and provide help for treating HSP. Methods:The vein blood samples were collected from 65 children with HSP and 30 normal children. The proportion of Th17 cells and regulatory T cells were tested by FCM and concentration of IL-17 and IL-23 in plasma were tested by ELISA. Results:Compared with normal children,the levels of Th17,Th17/Treg and IL-17,IL-23 were in increase at acute phase in children with HSP(P<0. 05). At recovery phase,that were in decrease compared with the acute phase(P<0. 05),but still higher than the normal children(P<0. 05). Compared with normal children,the level of Treg was in decrease at acute phase in children with HSP (P<0. 01). At recovery phase,that was in increase compared with the acute phase(P<0. 01),but still lower than the normal children (P<0. 01). Among the simplex,abdominal and other type of children with HSP,the levels of Th17,Treg,Th17/Treg and IL-17,IL-23 were same( P>0. 05 ) . At acute phase in children with HSP, Th17 cells percentage had positively correlated with IL-17 levels ( r=0. 880,P<0. 01),IL-23 levels had positively correlated with Th17 cells percentage and IL-17 levels (r=0. 838 or 0. 877,P<0. 01). Conclusion:Th17,Treg,Th17/Treg,IL-17 and IL-23 are involved in the course of the immunological pathogenesis in children with HSP,but the levels of that have no significant difference among simplex,abdominal and other types,further researches need to be done.
