Effect ofβ-sodium Aescinate on the Expression of Aquaporin-4 and Aquaporin-9 in Rats with Spinal Cord Injury
10.12007/j.issn.0258-4646.2016.12.015
- VernacularTitle:β-七叶皂甙钠对脊髓损伤大鼠脊髓组织水通道蛋白4与水通道蛋白9表达的影响
- Author:
Yong CHEN
;
Renjie CHI
- Keywords:
spinal cord injury;
β-sodium aescinate;
aquaporin-4;
aquaporin-9;
rat
- From:
Journal of China Medical University
2016;45(12):1124-1127,1132
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect ofβ?sodium aescinate on the expressions of aquaporin?4 and aquaporin?9 in rats with spinal cord inju?ry. Methods A total of 150 rats were randomly divided into 3 groups:sham group(n=50),spinal cord injury(SCI)group(n=50)andβ?sodi?um aescinate group(n=50). The experimental animal models was established by modified Allen’s model. The Basso,Beattie and Bresnahan (BBB)locomotor rating scale and inclined plane test were used to evaluate rat behavioral consequences after injury.The immunohistochemical staining and western blotting assay were performed to observe the expressions of aquaporin?4 and aquaporin?9. Results Compared with sham group,BBB score and inclined plane test score of SCI group andβ?sodium aescinate group were significantly lower at each time point(P<0.05);however,the functional recovery was significantly better inβ?sodium aescinate group than in SCI group at each time point from 7 d after SCI(P<0.05). The aquaporin?4 and aquaporin?9 positive expressions of rats in sham group were lower significantly than rats in SCI group andβ?sodium aescinate group(P<0.05);however,the aquaporin 4 and aquaporin 9 positive expressions of rats inβ?sodium aescinate group was lower signifi?cantly than rats in SCI group at each time point(P<0.05). Conclusion β?sodium aescinate can protect the neurologic function in rats with spi?nal cord injury by decreasing aquaporin?4 and aquaporin?9 protein expression.
