The investigation of the relationship between nucleo-tide-binding domain leucine-rich-containing family pyrin domain-containing-3 and the pathogenesis of collagen-induced arthritis
10.3760/cma.j.issn.1007-7480.2017.02.007
- VernacularTitle:核苷酸结合寡聚化结构域样受体3与胶原诱导性关节炎小鼠发病机制的探讨
- Author:
Yongfeng ZHANG
;
Yi ZHENG
- Keywords:
Arthritis;
Nucleo-tide-binding domain leucine-rich-containing family pyrin domaincontaining-3;
Pro-inflammatory cytokines
- From:
Chinese Journal of Rheumatology
2017;21(2):105-109,后插2
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between nucleo-tide-binding domain leucinerich-containing family pyrin domain-containing-3 (NLRP3) and the pathogenesis of arthritis.Methods The collagen-induced arthritis (CIA) mouse were divided into two groups:the model group (CIA,n=16) and the control group (n=8).The arthritis score and imaging evaluation (X-rays,Micro-CT) were performed;Synovial tissue NLRP3 expression were analyzed by immunohistochemical staining.Joint clinical score,radiographic score and joint NLRP3 expression were analyzed suing Pearson correlation analysis.Results The arthritis score (6.0±2.5),imaging score (4.6±0.9) and synovial tissue NLRP3 expression (4.0±2.0) were significantly increased in the CIA mice.The expression of synovial NLRP3 was positively correlated with arthritis clinical and radiographic scores (r=0.792,P＜0.05;r=0.669,P=0.005,respectively).Conclusion Synovial NLRP3 expression level is correlated with the clinical arthritis severity and extent of radiological destructions,suggesting that NLRP3 is involved in the pathogenesis of CIA.
