Effects of Carbamylated Erythropoietin on Coronary Microcirculation Endothelial Cells in Rats with Dia-betes Mellitus
10.6039/j.issn.1001-0408.2016.25.10
- VernacularTitle:氨甲酰促红细胞生成素对糖尿病模型大鼠冠脉微循环内皮细胞的影响Δ
- Author:
Yan HUANG
;
Kun ZENG
;
Biao XU
- Publication Type:Journal Article
- Keywords:
Carbamylated erythropoietin;
Diabetes mellitus;
Coronary microcirculation;
Endothelial cells;
Rat
- From:
China Pharmacy
2016;27(25):3488-3490,3491
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of carbamylated erythropoietin(CEPO)on cardiovascular microcirculation in rats with diabetes mellitus. METHODS:Rats were randomly divided into blank control group,model group,CEPO low-dose,medi-um-dose and high-dose groups(500,1 000,2 000 u/kg)with 12 in each group. The rats in the last 4 groups were reduced diabetes mellitus model. All rats were given relevant medicine intragastrically twice a week,coronary microcirculation endothelial cells were separated after consecutive 4 weeks. Enzyme-linked immunosorbent assay was conducted to detect levels of peripheral serum prosta-cyclin (PGI2),vasoconstrictor endothelin-1 (ET-1),angiotensin Ⅱ(AngⅡ) and von Willebrand factor (vWF) of rats in each group;in vitro CCK 8 test was used to detect endothelial cell activity(OD value);real-time quantitative polymerase chain reaction was adopted to detect proliferation-related genes(Ki67,p16),poptosis-related genes(Bad,Bax),and expressions of protein vas-cular endothelial growth factor(VEGF)and AngⅠ. RESULTS:Compared with blank control group,levels of PGI2,ET-1,AngⅡand vWF in serum in model group increased;OD value deceased;Ki67,p16,Bax and VEGF expression decreased;the difference was statistically significant (P<0.05). Compared with model group,levels of PGI2,ET-1,AngⅡ and vWF in serum in CEPO low-dose,medium-dose and high-dose groups increased;OD value increased;Ki67,p16 and VEGF expression increased;expres-sions of Bad and Bax decreased;the difference was statistically significant(P<0.05). The others had no significant difference(P>0.05). CONCLUSIONS:CEPO maybe improve the coronary microcirculation function by upregulating VEGF expression in coro-nary microcirculation endothelial cells and promoting endothelial cells’regeneration.
