Simultaneous Determination of Four Components in Compound Chuanxinlian Tablet by HPLC
10.6039/j.issn.1001-0408.2016.24.35
- VernacularTitle:HPLC法同时测定复方穿心莲片中4种成分的含量
- Author:
Shiyin ZHAN
- Publication Type:Journal Article
- Keywords:
HPLC;
Compound chuanxinlian tablet;
Andrographolide;
Quercetin-3-O-glucoside;
Dehydroandrographolide;
Kaempferol-3-O-glucoside
- From:
China Pharmacy
2016;27(24):3425-3427,3428
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of andrographolide,quercetin-3-O-gluco-side,dehydroandrographolide and kaempferol-3-O-glucoside in Compound chuanxinlian tablet. METHODS:HPLC was performed on the column of Phenomenex C18 with mobile phase of acetonitrile- 0.1% phosphoric acid aqueous(gradient elution)at a flow rate of 1.0 ml/min,the detection wavelength was 225 nm for andrographolide,335 nm for quercetin-3-O-glucoside and 254 nm for de-hydroandrographolide and kaempferol-3-O-glucoside,column temperature was 25 ℃,and the volume injection was 10 μl. RE-SULTS:The linear range was 0.111-2.770 μg for andrographolide(r=0.999 9),0.064-1.605 μg for quercetin-3-O-glucoside (r=0.999 9),0.086-2.138 μg for dehydroandrographolide(r=0.999 9)and 0.050-1.260 μg for kaempferol-3-O-glucoside(r=0.999 9);the limits of quantitation were 0.48 ng,0.88 ng,0.93 ng and 1.03 ng,the limits of detection were 0.79 ng,1.15 ng,0.96 ng and 1.07 ng,respectively;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 96.57%-100.30%(RSD=1.3%,n=9),95.04%-98.87%(RSD=1.3%,n=9),95.74%-102.92%(RSD=2.4%,n=9) and 96.73%-100.95%(RSD=1.6%,n=9),respectively. CONCLUSIONS:The method is sensitive,reliable and reproducible,and can be used for the si-multaneous determination of andrographolide,quercetin-3-O-glucoside,dehydroandrographolide and kaempferol-3-O-glucoside in Compound chuanxinlian tablet.
