Contents Determination of Ginsenoside Rg1,Re,Rb1 and Geniposide in Panax ginseng Processed by Gardenia jasminoides by HPLC
10.6039/j.issn.1001-0408.2016.24.43
- VernacularTitle:HPLC法测定栀制人参中人参皂苷Rg1、Re、Rb1和栀子苷的含量
- Author:
Yan QU
;
Tianzhu JIA
- Publication Type:Journal Article
- Keywords:
Panax ginseng processed by Gardenia jasminoides;
Ginsenoside;
Geniposide;
Content;
HPLC
- From:
China Pharmacy
2016;27(24):3449-3451,3452
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the contents determination of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and geniposide in Panax ginseng processed by Gardenia jasminoides. METHODS:HPLC was performed on the column of Agi-lent TC18 with mobile phase of acetonitrile-0.1% phosphoric acid,(gradient elution,ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1)and acetonitrile-water(13∶87,V/V,geniposide)at a flow rate of 1.0 ml/min,the detection wavelength was 203 nm for ginsen-oside Rg1,ginsenoside Re,ginsenoside Rb1 and 238 nm for geniposide,column temperature was 40 ℃,and injection volume was 20 μl. RESULTS:The linear range was 0.418-3.762 μg for ginsenoside Rg1(r=0.999 8),0.270-2.430 μg for Re(r=0.999 8), 0.398-3.582 μg for ginsenoside Rb1(r=0.999 7)and 0.606-3.030 μg for geniposide(r=0.999 7);RSDs of precision,stability and reproducibility tests were less than 2.0%;recoveries were 97.86%-101.47%(RSD=1.29%,n=6),96.21%-100.11%(RSD=1.42%,n=6),96.24%-100.48%(RSD=1.57%,n=6)and 97.76%-102.44%(RSD=1.71%,n=6). CONCLUSIONS:The meth-od is simple with good precision,stability and reproducibility,and can be used for the contents determination of ginsenoside Rg1, ginsenoside Re,ginsenoside Rb1 and geniposide in P. ginseng processed by Gardenia jasminoides.
