Study on Simultaneous Determination Method of Zolpidem,Dexzopiclone and Zaleplon in Human Plasma by UPLC-MS/MS
10.6039/j.issn.1001-0408.2016.23.09
- VernacularTitle:UPLC-MS/MS法同时检测人血浆中唑吡坦、右佐匹克隆和扎来普隆的方法研究
- Author:
Xin WEI
;
Jing AN
;
Yin WU
;
Zhanjun DONG
;
Liju FAN
;
Wanjun BAI
- Publication Type:Journal Article
- Keywords:
UPLC-MS/MS;
Zolpidem;
Dexzopiclone;
Zaleplon;
Determination
- From:
China Pharmacy
2016;27(23):3194-3196,3197
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a method for rapid,accurate and simultaneous determination of dexzopiclone,zolpidam and zaleplon in human plasma. METHODS:After the plasma sample was processed by liquid-liquid extraction,UPLC-MS/MS was established to detect plasma sample with carbamazepine as internal standard. The separation was performed on a Waters ACQUITY UPLC HSS T3 column with mobile phase composed of 0.1% ammonium solution-acetonitrile (gradient elution) at flow rate of 0.2 ml/min. The column temperature was set at 40 ℃,and sample size was 5 μl. The detection was performed by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source in positive mode. The mass transition ion-pairs were as follows:m/z 308.2→263.1(zolpidem),m/z 389.3→245.0(dexzopiclone),m/z 306.2→236.1(zaleplon),m/z 237.3→151.2(internal standard). RE-SULTS:The linear range of zolpidem,dexzopiclone and zaleplon were 0.02-20.00,0.50-20.00,0.02-20.00 ng/ml,respectively (r=0.990 1,0.996 8,0.991 7). LLOQ of them were 0.02,0.50,0.02 ng/ml,and detection limit were 0.01,0.20,0.01 ng/ml. RS-Ds of intra-day and inter-day were all lower than 15% ;extraction recovery were 88.9% -106.5% ;matrix effect were 94.8%-106.3%. CONCLUSIONS:The method is simple,rapid,sensitive and specific,and it can be used for simultaneous deter-mination of zaleplon,zolpidem and dexzopiclone in human plasma.
