Recombinant DNA and Protein Vaccines for Foot-and-mouth Disease Induce Humoral and Cellular Immune Responses in Mice.
- Author:
Ji young BAE
1
;
Sun Hwa MOON
;
Jung Ah CHOI
;
Jong Sug PARK
;
Bum Soo HAHN
;
Ki Yong KIM
;
Byunghan KIM
;
Jae Young SONG
;
Dae Hyuck KWON
;
Suk Chan LEE
;
Jong Bum KIM
;
Joo Sung YANG
Author Information
- Publication Type:Original Article
- Keywords: FMDV; DNA vaccine; Recombinant protein vaccine; B cell epitope peptide
- MeSH: Animals; Antibodies; Clone Cells; DNA; DNA, Recombinant; Enzyme-Linked Immunosorbent Assay; Enzyme-Linked Immunospot Assay; Epitopes, T-Lymphocyte; Foot-and-Mouth Disease; Foot-and-Mouth Disease Virus; Humans; Immunity, Cellular; Livestock; Mice; Picornaviridae; Proteins; RNA Replicase; RNA Viruses; Vaccines; Virion; Viruses
- From:Immune Network 2009;9(6):265-273
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Foot-and-mouth disease virus (FMDV) is a small single-stranded RNA virus which belongs to the family Picornaviridae, genus Apthovirus. It is a principal cause of FMD which is highly contagious in livestock. In a wild type virus infection, infected animals usually elicit antibodies against structural and non-structural protein of FMDV. A structural protein, VP1, is involved in neutralization of virus particle, and has both B and T cell epitopes. A RNA-dependent RNA polymerase, 3D, is highly conserved among other serotypes and strongly immunogenic, therefore, we selected VP1 and 3D as vaccine targets. METHODS: VP1 and 3D genes were codon-optimized to enhance protein expression level and cloned into mammalian expression vector. To produce recombinant protein, VP1 and 3D genes were also cloned into pET vector. The VP1 and 3D DNA or proteins were co-immunized into 5 weeks old BALB/C mice. RESULTS: Antigen-specific serum antibody (Ab) responses were detected by Ab ELISA. Cellular immune response against VP1 and 3D was confirmed by ELISpot assay. CONCLUSION: The results showed that all DNA- and protein-immunized groups induced cellular immune responses, suggesting that both DNA and recombinant protein vaccine administration efficiently induced Ag-specific humoral and cellular immune responses.
