The effects of HSPC238 on the expression of RB in hepatoma carcinoma cells
10.3969/j.issn.1673-4130.2016.18.001
- VernacularTitle:HSPC238对肝癌细胞中 RB基因表达的影响
- Author:
Chunlei YUAN
;
Jiayu TAN
;
Xiang HUANG
;
Jinglin CHEN
- Publication Type:Journal Article
- Keywords:
HSPC238;
RB gene;
hepatoma carcinoma;
HepG2
- From:
International Journal of Laboratory Medicine
2016;37(18):2505-2506,2510
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of up‐regulation and down‐regulation of HSPC238 on the RB gene mRNA and pRb protein .Methods PcDNA3 .1‐HSPC238 vector and PLL3 .7‐HSPC238 vector was transiently transfected into HepG2 cells re‐spectively .The level of RB mRNA was detected by real‐time PCR and pRb was detected by Western blotting .Results The level of RB mRNA and pRb in up‐regulation group both increased compared with control grops respectively .Conversely ,the level of RB mRNA and pRb in up‐regulation group both decreased compared with control grops respectively .Above results were all statistically significant(P<0 .05) .Conclusion HSPC238 could have a positive effect on the expression of the RB gene .
