Content Determination of Amygdalin in the Lianhua Qingwen Capsule by HPLC
10.6039/j.issn.1001-0408.2016.21.31
- VernacularTitle:HPLC法测定连花清瘟胶囊中苦杏仁苷的含量
- Author:
Dan BI
;
Yupeng CHEN
;
Shujing WANG
;
Guijin WANG
;
Qian ZHAO
;
Feng WEI
- Publication Type:Journal Article
- Keywords:
Lianhua qingwen capsule;
HPLC;
Amygdalin
- From:
China Pharmacy
2016;27(21):2978-2979,2980
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of amygdalin in Lianhua qingwen capsule. METH-ODS:HPLC was performed on the column of Phenomenex Kinetex XB-C18 with mobile phase of acetonitrile-0.2%Phosphoric acid so-lution(6∶94,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 207 nm,column temperature was 30℃,and the injec-tion volume was 10μl. RESULTS:The linear range of amygdalin was 43.16-215.80 μg/ml(r=0.999 7);the limit of detection was 0.431 6μg/ml,the limit of quantitation was 1.294 8μg/ml;RSDs of precision,stability and reproducibility tests no more than 0.69%;recovery was 95.16%-100.49%(RSD=1.67%,n=9). CONCLUSIONS:The method is simple and rapid with high accuracy and well reproducibility,and can be used for the content determination of amygdalin in Lianhua qingwen capsule.
