Simultaneous Determination of Imperatorin and Isoimperatorin in Qingxuan Tablet by UPLC
10.6039/j.issn.1001-0408.2016.21.33
- VernacularTitle:UPLC法同时测定清眩片中欧前胡素和异欧前胡素的含量
- Author:
Lin YANG
- Publication Type:Journal Article
- Keywords:
Qingxuant tablet;
UPLC;
Imperatorin;
Isoimperatorin
- From:
China Pharmacy
2016;27(21):2983-2984,2985
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of imperatorin and isoimperatorin in Qingxu-an tablet. METHODS:UPLC was performed on the column of ACQUITY UPLC BEH C18 with mobile phase of methanol-water (gradient elution)at a flow rate of 0.3 ml/min,the detection wavelength was 248 nm,column temperature was 30 ℃,and the in-jection volume was 3 μl. RESULTS:The linear range was 1.305-13.05 μg/ml for imperatorin(r=0.999 9)and 1.096-10.96 μg/ml for isoimperatorin(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 1%;recoveries were 97.45%-100.10%(RSD=1.00%,n=9)and 98.33%-101.20%(RSD=1.01%,n=9),respectively. CONCLUSIONS:The method is accurate,fast and reliable,and can be used for the simultaneous determination of imperatorin and isoimperatorin in Qingxuan tablet.
