Content Determination of Verbascoside in Naosaitong Honeyed Pill by HPLC

VernacularTitle:HPLC法测定脑塞通蜜丸中毛蕊花糖苷的含量
Author: Shuai BIAN ; Shiting YU ; Xueyuan BAI ; Daqing ZHAO ; Yu ZHAO ; Lili WANG
Publication Type:Journal Article
Keywords: Naosaitong honeyed pill; Verbascoside; HPLC
From: China Pharmacy 2016;27(21):2993-2994,2995
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To establish a method for the content determination of verbascoside in Naosaitong honeyed pill. METHODS:HPLC was performed on the column of Zorbax ODX with mobile phase of acetonitrile-0.1% phosphoric acid(16∶84, V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 334 nm,column temperature was 30 ℃,and the injection volume was 10 μl. RESULTS:The linear range of verbascoside was 0.200-1.000 μg(r=0.999 8);RSDs of precision,stability and repro-ducibility tests were lower than 1%;recovery was 95.16%-99.78%(RSD=1.80,n=6). CONCLUSIONS:The method is simple and accurate,and suitable for the content determination of verbascoside in Naosaitong honeyed pill.