Study on the Quality Standard of Embelia laeta
10.6039/j.issn.1001-0408.2016.21.43
- VernacularTitle:藏药酸藤果的质量标准研究
- Author:
Jie XING
;
Qianqian KONG
- Publication Type:Journal Article
- Keywords:
Embelia laeta;
TLC;
HPLC;
Quality standard
- From:
China Pharmacy
2016;27(21):3009-3010,3011
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the quality standard for Embelia laeta. METHODS:TLC was adopted for the qualitative identification. HPLC was adopted for the content determination of embelin:the column was Waters C18 with mobile phase consisted of methanol-5%methanoic acid(90∶10,V/V)at a flow rate of 0.9 ml/min,the detection wavelength was 288 nm,the column tem-perature was 40 ℃,nd the injection volume was 10 μl. RESULTS:The TLC of E. laeta showed clear spots and good separation. The linear range of embelin was 4.012-40.12 μg/ml(r=0.999 4);RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was 96.54%-99.57%(RSD=1.20%,n=6). CONCLUSIONS:The established standard can be used for the quality control of E. laeta.
